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PRMT1 Rabbit mAb (A4502)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PRMT1 Rabbit mAb (A4502)

Western blot analysis of various lysates using PRMT1 Rabbit mAb (A4502) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - PRMT1 Rabbit mAb (A4502)

Immunohistochemistry analysis of paraffin-embedded Rat brain using PRMT1 Rabbit mAb (A4502) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - PRMT1 Rabbit mAb (A4502)

Immunohistochemistry analysis of paraffin-embedded Human colon using PRMT1 Rabbit mAb (A4502) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - PRMT1 Rabbit mAb (A4502)

Immunofluorescence analysis of C6 cells using PRMT1 Rabbit mAb (A4502) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PRMT1 Rabbit mAb (A4502)

Immunofluorescence analysis of NIH-3T3 cells using PRMT1 Rabbit mAb (A4502) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PRMT1 Rabbit mAb (A4502)

Immunofluorescence analysis of U-2 OS cells using PRMT1 Rabbit mAb (A4502) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePRMT1 Rabbit mAb
Catalog No.A4502
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1066
This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRMT1 (Q99873).
SequenceMAAAEAANCIMENFVATLANGMSLQPPLEEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTG
Gene ID
Swiss Prot
SynonymsANM1; HCP1; IR1B4; HRMT1L2; PRMT1
Calculated MW42kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSW620, A549
Cellular locationCytoplasm, Nucleus, cytosol, nucleoplasm.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - PRMT1 Rabbit mAb (A4502)}

Western blot - PRMT1 Rabbit mAb (A4502)

Western blot analysis of various lysates using PRMT1 Rabbit mAb (A4502) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - PRMT1 Rabbit mAb (A4502)}

Immunohistochemistry - PRMT1 Rabbit mAb (A4502)

Immunohistochemistry analysis of paraffin-embedded Rat brain using PRMT1 Rabbit mAb (A4502) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - PRMT1 Rabbit mAb (A4502)}

Immunohistochemistry - PRMT1 Rabbit mAb (A4502)

Immunohistochemistry analysis of paraffin-embedded Human colon using PRMT1 Rabbit mAb (A4502) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - PRMT1 Rabbit mAb (A4502)}

Immunofluorescence - PRMT1 Rabbit mAb (A4502)

Immunofluorescence analysis of C6 cells using PRMT1 Rabbit mAb (A4502) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PRMT1 Rabbit mAb (A4502)}

Immunofluorescence - PRMT1 Rabbit mAb (A4502)

Immunofluorescence analysis of NIH-3T3 cells using PRMT1 Rabbit mAb (A4502) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PRMT1 Rabbit mAb (A4502)}

Immunofluorescence - PRMT1 Rabbit mAb (A4502)

Immunofluorescence analysis of U-2 OS cells using PRMT1 Rabbit mAb (A4502) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A4502? Please let us know so that we can cite the reference in this datasheet.

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Secondary Antibodies (26)