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Peroxiredoxin 5 (PRDX5) Rabbit mAb (A3954)

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Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Peroxiredoxin 5 (PRDX5) Rabbit mAb (A3954)

Western blot analysis of extracts of various cell lines, using Peroxiredoxin 5 (PRDX5) (PRDX5) Rabbit mAb (A3954) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10148 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product namePeroxiredoxin 5 (PRDX5) Rabbit mAb
Catalog No.A3954
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0873
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein interacts with peroxisome receptor 1 and plays an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. The use of alternate transcription start sites is thought to result in transcript variants that use different in-frame translational start codons to generate isoforms that are targeted to the mitochondrion (isoform L) or peroxisome/cytoplasm (isoform S). Multiple related pseudogenes have been defined for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human Peroxiredoxin 5 (PRDX5) (PRDX5) (P30044).
SequenceCSKTHLPGFVEQAEALKAKGVQVVACLSVNDAFVTGEWGRAHKAEGKVRLLADPTGAFGKETDLLLDDSLVSIFGNRRLKRFSMVVQDGIVKALNVEPDGT
Gene ID
Swiss Prot
SynonymsPLP; ACR1; B166; PRXV; PMP20; PRDX6; prx-V; SBBI10; AOEB166; HEL-S-55
Calculated MW22kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-549, U-251MG, Mouse lung, Mouse liver, Mouse kidney, Rat liver, Rat brain, Rat heart
Cellular locationCytoplasm, Mitochondrion, Peroxisome

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ABclonal:Western blot - Peroxiredoxin 5 (PRDX5) Rabbit mAb (A3954)}

Western blot - Peroxiredoxin 5 (PRDX5) Rabbit mAb (A3954)

Western blot analysis of extracts of various cell lines, using Peroxiredoxin 5 (PRDX5) (PRDX5) Rabbit mAb (A3954) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10148 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

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Secondary Antibodies (26)