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Phospho-BCAR1-Y410 Rabbit pAb (AP0789)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Phospho-BCAR1-Y410 Rabbit pAb (AP0789)

Western blot analysis of lysates from HeLa cells, using Phospho-BCAR1-Y410 Rabbit pAb (AP0789) at 1:5000 dilution. HeLa cells were treated by Pervanadate (1nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product namePhospho-BCAR1-Y410 Rabbit pAb
Catalog No.AP0789
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer.
ImmunogenA synthetic phosphorylated peptide around Y410 of human BCAR1 (NP_055382.2).
SequenceGVYAV
Gene ID
Swiss Prot
SynonymsCAS; CAS1; CASS1; CRKAS; P130Cas; Phospho-BCAR1-Y410
Calculated MW93kDa
Observed MW140kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa treated by Pervanadate
Cellular locationCell junction, Cytoplasm, focal adhesion
ABclonal:Western blot - Phospho-BCAR1-Y410 Rabbit pAb (AP0789)}

Western blot - Phospho-BCAR1-Y410 Rabbit pAb (AP0789)

Western blot analysis of lysates from HeLa cells, using Phospho-BCAR1-Y410 Rabbit pAb (AP0789) at 1:5000 dilution. HeLa cells were treated by Pervanadate (1nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)