Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human
Western blot analysis of lysates from Raji cells, using Phospho-BTK-Y223 Rabbit mAb (AP1136) at 1:500 dilution. Raji cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
| Product name | Phospho-BTK-Y223 Rabbit mAb |
|---|---|
| Catalog No. | AP1136 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC0307 |
| Immunogen | A synthetic phosphorylated peptide around Y223 of human BTK (Q06187). |
|---|---|
| Sequence | ALYDY |
| Gene ID | |
| Swiss Prot | |
| Synonyms | AT; ATK; BPK; XLA; IMD1; AGMX1; IGHD3; PSCTK1; Phospho-BTK-Y223 |
| Calculated MW | 76kDa |
| Observed MW | 77kDa |
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | Raji treated by Pervanadate |
| Cellular location | Cell membrane, Cytoplasm, Nucleus, Peripheral membrane protein. |
| Customer validation | Other(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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