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Phospho-Camk2-T286 Rabbit mAb (AP1386)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of various lysates, using Phospho-Camk2a-T286 antibody (AP1386) at 1:5000 dilution.Mouse brain cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	Phospho-Camk2-T286 Rabbit mAb
Catalog No.	AP1386
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC57709

Background

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2 )/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T286 of human Camk2.
Sequence	QETVD
Gene ID
Swiss Prot
Synonyms	CAMKA; MRD53; MRT63; CaMKIIalpha; CaMKIINalpha; CAM2; CAMK2; CAMKB; MRD54; CaMKIIbeta; CAMKD; CAMK; CAMKG; MRD59; CAMK-II
Calculated MW	54kDa
Observed MW	50kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:10000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Mouse brain
Cellular location	Cell junction, Presynaptic cell membrane, Synapse
Customer validation	WB(Mus musculus, Homo sapiens) Other(Mus musculus) IF(Mus musculus)