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Phospho-Camk2-T286 Rabbit mAb (AP1386)

Publication (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - Phospho-Camk2-T286 Rabbit mAb (AP1386)

Western blot analysis of various lysates, using Phospho-Camk2a-T286 antibody (AP1386) at 1:5000 dilution.Mouse brain cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product namePhospho-Camk2-T286 Rabbit mAb
Catalog No.AP1386
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC57709
The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2 )/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene.
ImmunogenA synthetic phosphorylated peptide around T286 of human Camk2.
SequenceQETVD
Gene ID
Swiss Prot
SynonymsCAMKA; MRD53; MRT63; CaMKIIalpha; CaMKIINalpha; CAM2; CAMK2; CAMKB; MRD54; CaMKIIbeta; CAMKD; CAMK; CAMKG; MRD59; CAMK-II
Calculated MW54kDa
Observed MW50kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain
Cellular locationCell junction, Presynaptic cell membrane, Synapse
Customer validation

WB(Mus musculus, Homo sapiens)

Other(Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Phospho-Camk2-T286 Rabbit mAb (AP1386)}

    Western blot - Phospho-Camk2-T286 Rabbit mAb (AP1386)

    Western blot analysis of various lysates, using Phospho-Camk2a-T286 antibody (AP1386) at 1:5000 dilution.Mouse brain cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AP1386? Please let us know so that we can cite the reference in this datasheet.