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Phospho-Camk2-T286 Rabbit pAb (AP0255)

Publications (9) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Camk2-T286 Rabbit pAb (AP0255)

Western blot analysis of lysates from Mouse brain, using Phospho-Camk2-T286 Rabbit pAb (AP0255) at 1:4000 dilution. Mouse brain cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product namePhospho-Camk2-T286 Rabbit pAb
Catalog No.AP0255
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene.
ImmunogenA synthetic phosphorylated peptide around T286 of human Camk2a (NP_741960.1).
SequenceQETVD
Gene ID
Swiss Prot
SynonymsCAMKA; MRD53; MRT63; CaMKIIalpha; CaMKIINalpha; Phospho-Camk2a-T286
Calculated MW54kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:4000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain
Cellular locationCell junction, presynaptic cell membrane, synapse.
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus)

Co-IP(Homo sapiens)

qPCR(Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - Phospho-Camk2-T286 Rabbit pAb (AP0255)}

Western blot - Phospho-Camk2-T286 Rabbit pAb (AP0255)

Western blot analysis of lysates from Mouse brain, using Phospho-Camk2-T286 Rabbit pAb (AP0255) at 1:4000 dilution. Mouse brain cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using AP0255? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)