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Phospho-Cdc6-S54 Rabbit mAb (AP1153)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Cdc6-S54 Rabbit mAb (AP1153) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (1 uM) at 37℃ for 20 hours. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product name	Phospho-Cdc6-S54 Rabbit mAb
Catalog No.	AP1153
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1693

Background

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S54 of human Cdc6 (Q99741).
Sequence	PLSPR
Gene ID
Swiss Prot
Synonyms	CDC18L; HsCDC6; MGORS5; HsCDC18; Phospho-Cdc6-S54
Calculated MW	63kDa
Observed MW	63kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3 treated by Paclitaxel, NIH/3T3 treated by Hydroxyurea
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Homo sapiens) IF(Homo sapiens)