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Phospho-Chk1-S280 Rabbit pAb (AP0017)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Chk1-S280 Rabbit pAb (AP0017)

Western blot analysis of lysates from 293T cells, using Phospho-Chk1-S280 Rabbit pAb (AP0017) at 1:600 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Phospho-Chk1-S280 Rabbit pAb (AP0017)

Immunohistochemistry analysis of paraffin-embedded Rat heart using Phospho-Chk1-S280 Rabbit pAb (AP0017) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-Chk1-S280 Rabbit pAb (AP0017)

Immunohistochemistry analysis of paraffin-embedded Human placenta using Phospho-Chk1-S280 Rabbit pAb (AP0017) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-Chk1-S280 Rabbit pAb (AP0017)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using Phospho-Chk1-S280 Rabbit pAb (AP0017) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product namePhospho-Chk1-S280 Rabbit pAb
Catalog No.AP0017
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around S280 of human Chk1 (NP_001265.2).
SequenceVTSGG
Gene ID
Swiss Prot
SynonymsCHK1; Phospho-Chk1-S280
Calculated MW54kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T
Cellular locationCytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - Phospho-Chk1-S280 Rabbit pAb (AP0017)}

Western blot - Phospho-Chk1-S280 Rabbit pAb (AP0017)

Western blot analysis of lysates from 293T cells, using Phospho-Chk1-S280 Rabbit pAb (AP0017) at 1:600 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Phospho-Chk1-S280 Rabbit pAb (AP0017)}

Immunohistochemistry - Phospho-Chk1-S280 Rabbit pAb (AP0017)

Immunohistochemistry analysis of paraffin-embedded Rat heart using Phospho-Chk1-S280 Rabbit pAb (AP0017) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-Chk1-S280 Rabbit pAb (AP0017)}

Immunohistochemistry - Phospho-Chk1-S280 Rabbit pAb (AP0017)

Immunohistochemistry analysis of paraffin-embedded Human placenta using Phospho-Chk1-S280 Rabbit pAb (AP0017) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-Chk1-S280 Rabbit pAb (AP0017)}

Immunohistochemistry - Phospho-Chk1-S280 Rabbit pAb (AP0017)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using Phospho-Chk1-S280 Rabbit pAb (AP0017) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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