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Phospho-Cytokeratin 18 (KRT18)-S33 Rabbit pAb (AP0263)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from HT-29 cells, using Phospho-Cytokeratin 18 (KRT18)-S33 Rabbit pAb (AP0263). HT-29 cells were treated by EGF.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product name	Phospho-Cytokeratin 18 (KRT18)-S33 Rabbit pAb
Catalog No.	AP0263
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Immunogen information

Immunogen	A phospho specific peptide corresponding to residues surrounding S33 of human KRT18
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	K18; CK-18; CYK18; Phospho-Cytokeratin 18 (KRT18)-S33
Calculated MW	48kDa
Observed MW	48kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HT-29 treated by EGF
Cellular location	Cytoplasm, Nucleus, nucleolus, perinuclear region.
Customer validation	WB(Homo sapiens)