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Phospho-ErbB4/HER4-Y1284 Rabbit pAb (AP0034)

Review (1)Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-ErbB4/HER4-Y1284 Rabbit pAb (AP0034)

Western blot analysis of lysates from MCF7 cells using Phospho-ErbB4/HER4-Y1284 Rabbit pAb (AP0034) at 1:700 dilution. MCF7 cells were treated by IGF (200 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Review
ABclonal: review for Phospho-ErbB4/HER4-Y1284 Rabbit pAb(AP0034)

Overview

Product namePhospho-ErbB4/HER4-Y1284 Rabbit pAb
Catalog No.AP0034
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
ImmunogenA synthetic phosphorylated peptide around Y1284 of human ErbB4/HER4 (NP_005226.1).
SequencePEYLS
Gene ID
Swiss Prot
SynonymsHER4; ALS19; p180erbB4; Phospho-ErbB4/HER4-Y1284
Calculated MW147kDa
Observed MW180kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMCF7 treated by IGF
Cellular locationCell membrane, Mitochondrion, Nucleus, Single-pass type I membrane protein
Customer validation

(C57BL6)

WB(Mus musculus)

IHC(Mus musculus)

IF(Mus musculus)

Documents

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ABclonal:Western blot - Phospho-ErbB4/HER4-Y1284 Rabbit pAb (AP0034)}

Western blot - Phospho-ErbB4/HER4-Y1284 Rabbit pAb (AP0034)

Western blot analysis of lysates from MCF7 cells using Phospho-ErbB4/HER4-Y1284 Rabbit pAb (AP0034) at 1:700 dilution. MCF7 cells were treated by IGF (200 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)