Phospho-H2AX-S139 Rabbit pAb (AP1267)

Encodes HTA5, a histone H2A protein. H2AX is a meiosis-specific isoform of histone H2A. Upon DSB formation, rapid accumulation of phosphorylated H2AX (gamma-H2AX) occurs around the break site. #H2AX foci accumulate in early G2. Immunolocalization studies in spread preparations of wild-type meiocytes at G2/early leptotene revealed the accumulation of numerous rather diffuse gamma-H2AX foci throughout the chromatin. However, their accumulation is not contemporaneous with that of AtSPO11-1. At 3 h post-S, no gamma-H2AX foci are detected. During the 3- to 5-h window when AtSPO11-1 foci rapidly disappear, there is an equally swift accumulation of gamma-H2AX to a maximum of >50 diffuse foci. The level of gammaH2AX then remains constant for a further 13 h before undergoing a gradual decrease to 10#Aei20 foci in the 18- to 24-h post-S period. By 30 h the foci have disappeared from the chromatin.