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Phospho-Histone H2A-S129 Rabbit pAb (AP1063)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-Histone H2A-S129 Rabbit pAb (AP1063) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-Histone H2A-S129 Rabbit pAb (AP1063) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product name	Phospho-Histone H2A-S129 Rabbit pAb
Catalog No.	AP1063
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Predicted to enable DNA binding activity. Involved in DNA repair; negative regulation of transcription by RNA polymerase II; and transfer RNA gene-mediated silencing. Part of replication fork protection complex. Orthologous to several human genes including H2AX (H2A.X variant histone).

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S129 of saccharomyces cerevisiae Histone H2A (NP_010511.3?).
Sequence	KASQE
Gene ID
Swiss Prot
Synonyms	H2A1; SPT11; Phospho-Histone H2A-S129
Calculated MW	14kDa
Observed MW

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Immunohistochemistry
Positive samples
Cellular location	nucleus