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Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb (AP1086)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb (AP1086)

Western blot analysis of lysates from 293T cells, using Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb (AP1086) at 1:1000 dilution or IGF1R antibody (A0243). 293T cells were treated by IGF-1 (50 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product namePhospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb
Catalog No.AP1086
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
ImmunogenA synthetic phosphorylated peptide around Y1165 & Y1166 of human IGF1RINSR (NP_000199.2).
SequenceYETDYY
Gene ID
Swiss Prot
SynonymsIGF1R/INSR; Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166
Calculated MW154kDa/155kDa/156kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T treated by IGF-1
Cellular locationcytoplasm, nucleus, plasma membrane, T-tubule.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb (AP1086)}

    Western blot - Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb (AP1086)

    Western blot analysis of lysates from 293T cells, using Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb (AP1086) at 1:1000 dilution or IGF1R antibody (A0243). 293T cells were treated by IGF-1 (50 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% BSA.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AP1086? Please let us know so that we can cite the reference in this datasheet.