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Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb (AP1086)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from 293T cells, using Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb (AP1086) at 1:1000 dilution or IGF1R antibody (A0243). 293T cells were treated by IGF-1 (50 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb
Catalog No.	AP1086
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y1165 & Y1166 of human IGF1RINSR (NP_000199.2).
Sequence	YETDYY
Gene ID
Swiss Prot
Synonyms	IGF1R/INSR; Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166
Calculated MW	154kDa/155kDa/156kDa
Observed MW	100kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293T treated by IGF-1
Cellular location	cytoplasm, nucleus, plasma membrane, T-tubule.
Customer validation	WB(Mus musculus)