제품 > 항체 > 인산화 특이적 항체

Phospho-JunB-S259 Rabbit pAb (AP0375)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-JunB-S259 Rabbit pAb (AP0375)

Western blot analysis of lysates from HT29 cells, using Phospho-JunB-S259 Rabbit pAb (AP0375).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product namePhospho-JunB-S259 Rabbit pAb
Catalog No.AP0375
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables sequence-specific double-stranded DNA binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in nucleoplasm. Part of transcription factor AP-1 complex. Biomarker of Hodgkin's lymphoma and anaplastic large cell lymphoma.
ImmunogenA phospho specific peptide corresponding to residues surrounding S259 of human Phospho-JunB-S259
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsAP-1; Phospho-JunB-S259
Calculated MW36kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHT-29, HT-29+EGF
Cellular locationNucleus
Customer validation

WB(Mus musculus)

IF(Mus musculus)

ABclonal:Western blot - Phospho-JunB-S259 Rabbit pAb (AP0375)}

Western blot - Phospho-JunB-S259 Rabbit pAb (AP0375)

Western blot analysis of lysates from HT29 cells, using Phospho-JunB-S259 Rabbit pAb (AP0375).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using AP0375? Please let us know so that we can cite the reference in this datasheet.

항체 (4)

Secondary Antibodies (26)