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Phospho-MLKL-S345 Rabbit pAb (AP1255)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of lysates from L929 cells, using Phospho-MLKL-S345 Rabbit pAb (AP1255) at 1:400 dilution. L929 cells were treated by 20 ng/ml TNF alpha100 nM Smac mimeticand 20 μM z-VAD for 8h
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name	Phospho-MLKL-S345 Rabbit pAb
Catalog No.	AP1255
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S345 of mouse MLKL (NP_001297542.1).
Sequence	TQNSIS
Gene ID
Swiss Prot
Synonyms	9130019I15Rik; Phospho-MLKL-S345
Calculated MW	54kDa
Observed MW	54kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	L929 treated by TNF alpha and z-VAD
Cellular location	Cell membrane, Cytoplasm.