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Phospho-PDHA1-S293 Rabbit mAb (AP1022)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa cells, using Phospho-PDHA1-S293 Rabbit mAb (AP1022) at 1:23000 dilution.293T cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from HeLa cells, using Phospho-PDHA1-S293 Rabbit mAb (AP1022) at1:23000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunoprecipitation - Phospho-PDHA1-S293 Rabbit mAb (AP1022)

Immunoprecipitation analysis of 300 μg extracts of NIH/3T3 cells using 3 μg Phospho-PDHA1-S293 antibody (AP1022). Western blot was performed from the immunoprecipitate using Phospho-PDHA1-S293 antibody (AP1022) at a dilution of 1:1000.

Overview

Product name	Phospho-PDHA1-S293 Rabbit mAb
Catalog No.	AP1022
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC53489

Background

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S293 of human PDHA1 (P08559).
Sequence	GHSMSD
Gene ID
Swiss Prot
Synonyms	PDHA; PDHAD; PHE1A; E1alpha; PDHCE1A; Phospho-PDHA1-S293
Calculated MW	43kDa
Observed MW	43kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:20000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	NIH/3T3, 293T
Cellular location	Mitochondrion matrix
Customer validation	WB(Homo sapiens, Rattus norvegicus) IHC(Homo sapiens) IP(Homo sapiens)