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Phospho-PKC alpha-T410+PKC delta-T410 Rabbit mAb (AP1506)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-PKC alpha-T410+PKC delta-T410 Rabbit mAb (AP1506)

Western blot analysis of lysates from wild type (WT)、 293F cells were treated by PMA、293F cells were treated by PMA after transfected with PRKCA and 293F cells were treated by PMA after transfected with PRKCD using Phospho-PKC alpha-T410+PKC delta-T410 Rabbit mAb (AP1506) at 1:2000 dilution. 293F、293F-PRKCA-Transfected and 293F-PRKCD-Transfected cells were treated by PMA (200 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product namePhospho-PKC alpha-T410+PKC delta-T410 Rabbit mAb
Catalog No.AP1506
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63068
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
ImmunogenA synthetic phosphorylated peptide around T410 of human PRKCZ(NP_002735.3).
SequenceTTSTFC
Gene ID
Swiss Prot
SynonymsAAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha; Phospho-PKC alpha-T410+PKC delta-T410
Calculated MW76kDa/77kDa
Observed MW76-80kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:3000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293F treated by PMA after transfected with PRKCA, 293F treated by PMA after transfected with PRKCD
Cellular locationCell junction, Cytoplasm, Endosome

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Phospho-PKC alpha-T410+PKC delta-T410 Rabbit mAb (AP1506)}

    Western blot - Phospho-PKC alpha-T410+PKC delta-T410 Rabbit mAb (AP1506)

    Western blot analysis of lysates from wild type (WT)、 293F cells were treated by PMA、293F cells were treated by PMA after transfected with PRKCA and 293F cells were treated by PMA after transfected with PRKCD using Phospho-PKC alpha-T410+PKC delta-T410 Rabbit mAb (AP1506) at 1:2000 dilution. 293F、293F-PRKCA-Transfected and 293F-PRKCD-Transfected cells were treated by PMA (200 nM) at 37℃ for 30 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)