Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from HeLa cells, using Phospho-PLC beta 3 (PLCB3)-S1105 Rabbit pAb (AP0837) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
| Product name | Phospho-PLC beta 3 (PLCB3)-S1105 Rabbit pAb |
|---|---|
| Catalog No. | AP0837 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around S1105 of human PLC beta 3 (PLCB3) (NP_000923.1). |
|---|---|
| Sequence | HNSIS |
| Gene ID | |
| Swiss Prot | |
| Synonyms | SMDCD; Phospho-PLC beta 3 (PLCB3)-S1105 |
| Calculated MW | 139kDa |
| Observed MW | 150kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HeLa treated by PMA |
| Cellular location | Membrane, Peripheral membrane protein. |
* For research use only. Not for therapeutic or diagnostic purposes.
_WB_01.jpg?t=1718355604 "ABclonal:Western blot - Phospho-PLC beta 3 (PLCB3)-S1105 Rabbit pAb (AP0837)")
