Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human
Western blot analysis of extracts of HT-29 cells, using Phospho-PLK1-T210 (AP1025) at 1:1000 dilution.HT-29 cells were treated by nocodazole (100 ng/mL) at 37℃ for 16 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
| Product name | Phospho-PLK1-T210 Rabbit mAb |
|---|---|
| Catalog No. | AP1025 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC1570 |
| Immunogen | A synthetic phosphorylated peptide around T210 of human PLK1 (P53350). |
|---|---|
| Sequence | KKTLC |
| Gene ID | |
| Swiss Prot | |
| Synonyms | PLK; STPK13 |
| Calculated MW | 68kDa |
| Observed MW | 68kDa |
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HT-29 treated by nocodazole |
| Cellular location | Chromosome, Cytoplasm, Midbody, Nucleus, Centromere, Centrosome, Cytoskeleton, Kinetochore, Microtubule organizing center, Spindle |
| Customer validation | WB(Homo sapiens) |
To download a Certificate of Compliance, please enter your Lot number below:
Lot number
* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using AP1025? Please let us know so that we can cite the reference in this datasheet.
