Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using Phospho-PPIG-S376 Rabbit pAb (AP0842) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
| Product name | Phospho-PPIG-S376 Rabbit pAb |
|---|---|
| Catalog No. | AP0842 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around S376 of human PPIG (NP_004783.2). |
|---|---|
| Sequence | VSSGE |
| Gene ID | |
| Swiss Prot | |
| Synonyms | CYP; SRCyp; SCAF10; CARS-Cyp; Phospho-PPIG-S376 |
| Calculated MW | 89kDa |
| Observed MW | 110kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | 293T, C2C12, PC-12 |
| Cellular location | Nucleus matrix, Nucleus speckle |
* For research use only. Not for therapeutic or diagnostic purposes.
_WB_01.jpg?t=1709544451 "ABclonal:Western blot - Phospho-PPIG-S376 Rabbit pAb (AP0842)")
