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Phospho-Rac1/2/3-S71 Rabbit pAb (AP0086)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Western blot analysis of lysates from A-431 cells, using Phospho-Rac1/2/3-S71 Rabbit pAb (AP0086) at 1:1000 dilution.A-431 cells were treated by EGF (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product name	Phospho-Rac1/2/3-S71 Rabbit pAb
Catalog No.	AP0086
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S71 of human RAC1 (NP_008839.2).
Sequence	RPLSYP
Gene ID
Swiss Prot
Synonyms	MIG5; MRD48; Rac-1; TC-25; p21-Rac1; Phospho-Rac1/2/3-S71
Calculated MW	21kDa
Observed MW	28kDa

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	A-431 treated by EGF
Cellular location	Cell membrane, Cytoplasm, Cytoplasmic side, Lipid-anchor, Melanosome, Nucleus
Customer validation	WB(Homo sapiens, Mus musculus)