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Phospho-Raptor-Ser792 Rabbit pAb (AP0928)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from 293T cells, using Phospho-Raptor-Ser792 Rabbit pAb (AP0928) at 1:1000 dilution. 293T cells were treated by Oligomycin (0. 5 uM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1S.

Overview

Product name	Phospho-Raptor-Ser792 Rabbit pAb
Catalog No.	AP0928
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S792 of human RPTOR (NP_065812.1).
Sequence	ASSYS
Gene ID
Swiss Prot
Synonyms	KOG1; Mip1; Phospho-Raptor-Ser792
Calculated MW	149kDa
Observed MW	Refer to figures

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293T treated by Oligomycin
Cellular location	Cytoplasm, Cytoplasmic granule, Lysosome.
Customer validation	WB(Mus musculus, Oryctolagus cuniculus) IHC(Ctenopharyngodon idellus) WB(Ctenopharyngodon idellus)