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Phospho-STK3/STK4-T180/T183 Rabbit pAb (AP1094)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using Phospho-STK3/STK4-T180/T183 Rabbit pAb (AP1094) at 1:1000 dilution. NIH/3T3 cells and C6 cells were treated by Staurosporine(1uM) at room temperature for 3 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	Phospho-STK3/STK4-T180/T183 Rabbit pAb
Catalog No.	AP1094
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T180 of human STK3/STK4STK3 (NP_006272.2).
Sequence	RNTVI
Gene ID
Swiss Prot
Synonyms	KRS1; MST2; KRS2; MST1; YSK3
Calculated MW	56kDa
Observed MW	59kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3 treated by Staurosporine, C6 treated by Staurosporine
Cellular location	cytoplasm, cytosol, nucleus.
Customer validation	WB(Drosophila melanogaster, Mus musculus)