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Proteasome 20S LMP7/PSMB8 Rabbit mAb (A23378)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using Proteasome 20S LMP7/PSMB8 Rabbit mAb (A23378) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Proteasome 20S LMP7/PSMB8 Rabbit mAb (A23378)

Immunohistochemistry analysis of Proteasome 20S LMP7/PSMB8 in paraffin-embedded mouse intestin tissue using Proteasome 20S LMP7/PSMB8 Rabbit mAb (A23378) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Proteasome 20S LMP7/PSMB8 in paraffin-embedded rat liver tissue using Proteasome 20S LMP7/PSMB8 Rabbit mAb (A23378) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Proteasome 20S LMP7/PSMB8 in paraffin-embedded rat colon tissue using Proteasome 20S LMP7/PSMB8 Rabbit mAb (A23378) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Proteasome 20S LMP7/PSMB8 in paraffin-embedded human liver tissue using Proteasome 20S LMP7/PSMB8 Rabbit mAb (A23378) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product name	Proteasome 20S LMP7/PSMB8 Rabbit mAb
Catalog No.	A23378
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC60121

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.

Immunogen information

Immunogen	Recombinant protein of human Proteasome 20S LMP7/PSMB8
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	JMP; ALDD; LMP7; NKJO; D6S216; PRAAS1; PSMB5i; RING10; D6S216E; Proteasome 20S LMP7/PSMB8
Calculated MW	30kDa
Observed MW	23kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:6000 IHC-P 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	Jurkat, Raji, 293F(Negative control), RAW264.7, Mouse thymus, PC-12, Rat thymus
Cellular location	Cytoplasm, Nucleus