제품 > 항체 > 다중클론항체(pAb)

QDPR Rabbit pAb (A5733)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - QDPR Rabbit pAb (A5733)

Western blot analysis of extracts of various cell lines, using QDPR antibody (A5733) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - QDPR Rabbit pAb (A5733)

Western blot analysis of extracts of various cell lines, using QDPR antibody (A5733) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

Overview

Product nameQDPR Rabbit pAb
Catalog No.A5733
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the enzyme dihydropteridine reductase, which catalyzes the NADH-mediated reduction of quinonoid dihydrobiopterin. This enzyme is an essential component of the pterin-dependent aromatic amino acid hydroxylating systems. Mutations in this gene resulting in QDPR deficiency include aberrant splicing, amino acid substitutions, insertions, or premature terminations. Dihydropteridine reductase deficiency presents as atypical phenylketonuria due to insufficient production of biopterin, a cofactor for phenylalanine hydroxylase.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human QDPR (NP_000311.2).
SequenceMAAAAAAGEARRVLVYGGRGALGSRCVQAFRARNWWVASVDVVENEEASASIIVKMTDSFTEQADQVTAEVGKLLGEEKVDAILCVAGGWAGGNAKSKSLFKNCDLMWKQSIWTSTISSHLATKHLKEGGLLTLAGAKAALDGTPGMIGYGMAKGAVHQLCQSLAGKNSGMPPGAAAIAVLPVTLDTPMNRKSMPEADFSSWTPLEFLVETFHDWITGKNRPSSGSLIQVVTTEGRTELTPAYF
Gene ID
Swiss Prot
SynonymsDHPR; PKU2; HDHPR; SDR33C1
Calculated MW26kDa
Observed MW26kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse liver, Mouse kidney, Mouse brain, Rat liver, HL-60, BT-474, SW620
Cellular locationcytoplasm, cytosol, extracellular exosome
Customer validation

WB(Homo sapiens, Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - QDPR Rabbit pAb (A5733)}

Western blot - QDPR Rabbit pAb (A5733)

Western blot analysis of extracts of various cell lines, using QDPR antibody (A5733) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - QDPR Rabbit pAb (A5733)}

Western blot - QDPR Rabbit pAb (A5733)

Western blot analysis of extracts of various cell lines, using QDPR antibody (A5733) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A5733? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)