Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using RAB7 Rabbit mAb (A12308) at 1:55000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
Confocal imaging of NIH/3T3 cells using RAB7 Rabbit mAb (A12308, dilution 1:100) followed by a further incubation with ABflo® 488-conjugated Goat Anti-Rabbit IgG (H+L) (AS073, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with Cy3 Goat Anti-Mouse IgG (H+L) Ab (AS008, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
| Product name | RAB7 Rabbit mAb |
|---|---|
| Catalog No. | A12308 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC54437 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 108-207 of human RAB7 (NP_004628.4). |
|---|---|
| Sequence | IQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAKASAESCSC |
| Gene ID | |
| Swiss Prot | |
| Synonyms | RAB7; CMT2B; PRO2706 |
| Calculated MW | 23kDa |
| Observed MW | 23kDa/ |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunofluorescence |
| Positive samples | mouse brain, Hela, Neuro-2a, C6, |
| Cellular location | Cytoplasmic side, Cytoplasmic vesicle, Late endosome membrane, Lipid droplet, Lysosome membrane, Melanosome membrane, Peripheral membrane protein, autophagosome membrane, phagosome membrane. |
| Customer validation | WB(Homo sapiens, Sus scrofa, Mus musculus) IF(Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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