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RBM14 Rabbit pAb (A13159)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RBM14 Rabbit pAb (A13159)

Western blot analysis of various lysates using RBM14 Rabbit pAb (A13159) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - RBM14 Rabbit pAb (A13159)

Immunofluorescence analysis of NIH/3T3 cells using RBM14 Rabbit pAb (A13159) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RBM14 Rabbit pAb (A13159)

Immunofluorescence analysis of U2OS cells using RBM14 Rabbit pAb (A13159) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRBM14 Rabbit pAb
Catalog No.A13159
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a ribonucleoprotein that functions as a general nuclear coactivator, and an RNA splicing modulator. This protein contains two RNA recognition motifs (RRM) at the N-terminus, and multiple hexapeptide repeat domain at the C-terminus that interacts with thyroid hormone receptor-binding protein (TRBP), and is required for transcription activation. Alternatively spliced transcript variants encoding different isoforms (with opposing effects on transcription) have been described for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 110-220 of human RBM14 (NP_006319.1).
SequenceVVKDYAFVHMEKEADAKAAIAQLNGKEVKGKRINVELSTKGQKKGPGLAVQSGDKTKKPGAGDTAFPGTGGFSATFDYQQAFGNSTGGFDGQARQPTPPFFGRDRSPLRRS
Gene ID
Swiss Prot
SynonymsSIP; COAA; PSP2; SYTIP1; TMEM137; RBM14
Calculated MW69kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, 293T, Jurkat, Rat testis
Cellular locationNucleus, nucleolus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - RBM14 Rabbit pAb (A13159)}

Western blot - RBM14 Rabbit pAb (A13159)

Western blot analysis of various lysates using RBM14 Rabbit pAb (A13159) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - RBM14 Rabbit pAb (A13159)}

Immunofluorescence - RBM14 Rabbit pAb (A13159)

Immunofluorescence analysis of NIH/3T3 cells using RBM14 Rabbit pAb (A13159) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RBM14 Rabbit pAb (A13159)}

Immunofluorescence - RBM14 Rabbit pAb (A13159)

Immunofluorescence analysis of U2OS cells using RBM14 Rabbit pAb (A13159) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)