제품 > 항체 > 다중클론항체(pAb)

RNF138 Rabbit pAb (A10304)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - RNF138 Rabbit pAb (A10304)

Western blot analysis of extracts of BT-474 cells, using RNF138 antibody (A10304) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameRNF138 Rabbit pAb
Catalog No.A10304
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable protein kinase binding activity; single-stranded DNA binding activity; and ubiquitin protein ligase activity. Acts upstream of or within cellular response to leukemia inhibitory factor. Predicted to be located in chromosome. Predicted to be active in site of double-strand break. Is expressed in several structures, including genitourinary system; lung; nervous system; sensory organ; and tooth. Orthologous to human RNF138 (ring finger protein 138).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 60-245 of mouse RNF138 (Q9CQE0).
SequenceSVTRRERACPERALDLENIMRRFSGSCRCCSKKIKFYRMRHHYKSCKKYQDEYGVSSVIPNFKISQDSVRSSNRSETSASDNTETYQEDTSSSGHPTFKCPLCQESNFTRQRLLDHCNSNHLFQIVPVTCPICVSLPWGDPSQITRNFVSHLNQRHQFDYGEFVNLQLDEETQYQTAVEESFQVNM
Gene ID
Swiss Prot
SynonymsTrif; STRIN; Trif-d; 2410015A17Rik; 2810480D20Rik
Calculated MW24kDa/28kDa
Observed MW28kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesBT-474
Cellular location
Customer validation

WB(Sus scrofa, Mus musculus)

    ABclonal:Western blot - RNF138 Rabbit pAb (A10304)}

    Western blot - RNF138 Rabbit pAb (A10304)

    Western blot analysis of extracts of BT-474 cells, using RNF138 antibody (A10304) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A10304? Please let us know so that we can cite the reference in this datasheet.