제품 > 항체 > 다중클론항체(pAb)

ROS1 Rabbit pAb (A16669)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ROS1 Rabbit pAb (A16669)

Western blot analysis of extracts of mouse brain, using ROS1 antibody (A16669) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

Overview

Product nameROS1 Rabbit pAb
Catalog No.A16669
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1934-2232 of human ROS1 (NP_002935.2?).
SequenceIENLPAFPREKLTLRLLLGSGAFGEVYEGTAVDILGVGSGEIKVAVKTLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLGVCLLNEPQYIILELMEGGDLLTYLRKARMATFYGPLLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDIYKNDYYRKRGEGLLPVRWMAPESLMDGIFTTQSDVWSFGILIWEILTLGHQPYPAHSNLDVLNYVQTGGRLEPPRNCPDDLWNLMTQCWAQEPDQRPTFHRIQDQLQLFRNFFLNSIYKSRDE
Gene ID
Swiss Prot
SynonymsROS; MCF3; c-ros-1
Calculated MW264kDa
Observed MW263kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain
Cellular locationplasma membrane
Customer validation

WB(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - ROS1 Rabbit pAb (A16669)}

Western blot - ROS1 Rabbit pAb (A16669)

Western blot analysis of extracts of mouse brain, using ROS1 antibody (A16669) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A16669? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)