제품 > 항체 > 다중클론항체(pAb)

RPB3/POLR2C Rabbit pAb (A1785)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using RPB3/POLR2C Rabbit pAb (A1785) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunoprecipitation - RPB3/POLR2C Rabbit pAb (A1785)

Immunoprecipitation analysis of 150 μg extracts of SW620 cells using 3 μg RPB3/POLR2C antibody (A1785). Western blot was performed from the immunoprecipitate using RPB3/POLR2C antibody (A1785) at a dilution of 1:500.

Overview

Product name	RPB3/POLR2C Rabbit pAb
Catalog No.	A1785
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human RPB3/POLR2C (NP_116558.1).
Sequence	MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGLIPLISDDIVDKLQYSRDCTCEEFCPECSVEFTLDVRCNEDQTRHVTSRDLISNSPRVIPVTSRNRDNDPNDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPTAGVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQAPYDPNGKPERFYYNVESCGSLRPETIVLSALSGLKKKLSDLQTQLSHEIQSDVLTIN
Gene ID
Swiss Prot
Synonyms	RPB3; RPB31; hRPB33; hsRPB3; RPB3/POLR2C
Calculated MW	31kDa
Observed MW	38kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	SW620, OVCAR3, Mouse spleen
Cellular location	Nucleus