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RPS8 Rabbit pAb (A18377)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RPS8 Rabbit pAb (A18377)

Western blot analysis of various lysates using RPS8 Rabbit pAb (A18377) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - RPS8 Rabbit pAb (A18377)

Immunofluorescence analysis of L929 cells using RPS8 Rabbit pAb (A18377) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPS8 Rabbit pAb (A18377)

Immunofluorescence analysis of C6 cells using RPS8 Rabbit pAb (A18377) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameRPS8 Rabbit pAb
Catalog No.A18377
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S8E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal tumors and colon polyps compared to matched normal colonic mucosa has been observed. This gene is co-transcribed with the small nucleolar RNA genes U38A, U38B, U39, and U40, which are located in its fourth, fifth, first, and second introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 10-100 of human RPS8 (NP_001003.1).
SequenceKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGGNKKYRALRLDVGNFSWGSECCTRKTRIIDVVYNASNNELVRTKTLVKNC
Gene ID
Swiss Prot
SynonymsS8; eS8; RPS8
Calculated MW24kDa
Observed MW24kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesSGC-7901, HeLa, Mouse pancreas, Mouse ovary, Rat pancreas
Cellular locationcytoplasm, cytosol, cytosolic ribosome, endoplasmic reticulum, extracellular exosome, focal adhesion, nucleoplasm, nucleus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - RPS8 Rabbit pAb (A18377)}

Western blot - RPS8 Rabbit pAb (A18377)

Western blot analysis of various lysates using RPS8 Rabbit pAb (A18377) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - RPS8 Rabbit pAb (A18377)}

Immunofluorescence - RPS8 Rabbit pAb (A18377)

Immunofluorescence analysis of L929 cells using RPS8 Rabbit pAb (A18377) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RPS8 Rabbit pAb (A18377)}

Immunofluorescence - RPS8 Rabbit pAb (A18377)

Immunofluorescence analysis of C6 cells using RPS8 Rabbit pAb (A18377) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A18377? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)