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Recoverin Rabbit mAb (A25214)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Recoverin Rabbit mAb (A25214)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Recoverin using Recoverin Rabbit mAb (A25214) at 1:10000 dilution. 
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 40.5 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Recoverin Rabbit mAb (A25214)

Immunohistochemistry analysis of paraffin-embedded Rat retina tissue using Recoverin Rabbit mAb (A25214) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Recoverin Rabbit mAb (A25214)

Immunohistochemistry analysis of paraffin-embedded Mouse retina tissue using Recoverin Rabbit mAb (A25214) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameRecoverin Rabbit mAb
Catalog No.A25214
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC65841
This gene encodes a member of the recoverin family of neuronal calcium sensors. The encoded protein contains three calcium-binding EF-hand domains and may prolong the termination of the phototransduction cascade in the retina by blocking the phosphorylation of photo-activated rhodopsin. Recoverin may be the antigen responsible for cancer-associated retinopathy.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Recoverin (NP_002894.1).
SequenceMGNSKSGALSKEILEELQLNTKFSEEELCSWYQSFLKDCPTGRITQQQFQSIYAKFFPDTDPKAYAQHVFRSFDSNLDGTLDFKEYVIALHMTTAGKTNQKLEWAFSLYDVDGNGTISKNEVLEIVMAIFKMITPEDVKLLPDDENTPEKRAEKIWKYFGKNDDDKLTEKEFIEGTLANKEILRLIQFEPQKVKEKMKNA
Gene ID
Swiss Prot
SynonymsRCV1; RCVRN
Calculated MW23kDa
Observed MW25kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:40000
  • IHC-P 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293F transfected with Recoverin (Human)
Cellular locationPhotoreceptor outer segment membrane, perikaryon.

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ABclonal:Western blot - Recoverin Rabbit mAb (A25214)}

Western blot - Recoverin Rabbit mAb (A25214)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Recoverin using Recoverin Rabbit mAb (A25214) at 1:10000 dilution. 
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 40.5 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Recoverin Rabbit mAb (A25214)}

Immunohistochemistry - Recoverin Rabbit mAb (A25214)

Immunohistochemistry analysis of paraffin-embedded Rat retina tissue using Recoverin Rabbit mAb (A25214) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Recoverin Rabbit mAb (A25214)}

Immunohistochemistry - Recoverin Rabbit mAb (A25214)

Immunohistochemistry analysis of paraffin-embedded Mouse retina tissue using Recoverin Rabbit mAb (A25214) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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