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SAFB Rabbit mAb (A21125)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SAFB Rabbit mAb (A21125)

Western blot analysis of various lysates using SAFB Rabbit mAb (A21125) at1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameSAFB Rabbit mAb
Catalog No.A21125
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2983
This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 815-915 of human SAFB (NP_002958.2).
SequencePPPRRDWGDHGRREDDRSWQGTADGGMMDRDHKRWQGGERSMSGHSGPGHMMNRGGMSGRGSFAPGGASRGHPIPHGGMQGGFGGQSRGSRPSDARFTRRY
Gene ID
Swiss Prot
SynonymsHAP; HET; SAF-B; SAFB1; SAB-B1; SAF-B1; SAFB
Calculated MW103kDa
Observed MW150kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, 293T, SH-SY5Y, Mouse thymus
Cellular locationNucleus.

Documents

Certificate of Compliance

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ABclonal:Western blot - SAFB Rabbit mAb (A21125)}

Western blot - SAFB Rabbit mAb (A21125)

Western blot analysis of various lysates using SAFB Rabbit mAb (A21125) at1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)}

Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)}

Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)}

Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)}

Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)}

Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)}

Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)}

Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)}

Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)}

Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SAFB Rabbit mAb (A21125)}

Immunohistochemistry - SAFB Rabbit mAb (A21125)

Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using SAFB Rabbit mAb (A21125) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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