제품 > 항체 > 다중클론항체(pAb)

SARS-CoV-2 N Protein Rabbit pAb (A20607)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:SARS-CoV-2

Western blot analysis of Recombinant SARS-CoV Nucleocapsid Protein (RP01296LQ), using SARS-CoV-2 N Protein Rabbit pAb (A20607) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 100ng/10ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name	SARS-CoV-2 N Protein Rabbit pAb
Catalog No.	A20607
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host SMAD3.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of coronavirus N Protein (NP_828858.1).
Sequence	MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDG
Gene ID
Swiss Prot
Synonyms
Calculated MW	46kDa
Observed MW	55kDa

Applications

Reactivity	SARS-CoV-2
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Recombinant SARS-CoV Nucleocapsid Protein
Cellular location