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SCN4B Rabbit pAb (A2154)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SCN4B Rabbit pAb (A2154)

Western blot analysis of various lysates using SCN4B Rabbit pAb (A2154) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameSCN4B Rabbit pAb
Catalog No.A2154
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables voltage-gated sodium channel activity. Acts upstream of or within sodium ion transmembrane transport. Located in intercalated disc. Is expressed in brain. Human ortholog(s) of this gene implicated in long QT syndrome 10. Orthologous to human SCN4B (sodium voltage-gated channel beta subunit 4).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 80-228 of mouse SCN4B (Q7M729).
SequenceIDGIVKNDKSDPKVRVKDDDRITLEGSTKEKTNNISILLSDLEFSDTGRYTCFVRNPKEKDLNNSATIFLQVVDKLEKVDNTVTLIILAVVGGVIGLLVCILLLKKLITFILKKTREKKKECLVSSSGNDNTENGLPGSKAEEKPPTKV
Gene ID
Swiss Prot
SynonymsGm1471; SCN4B
Calculated MW25kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, Mouse brain, Rat spinal cord
Cellular locationCell membrane, Single-pass type I membrane protein
Customer validation

WB(Mus musculus)

IF(Mus musculus)

    ABclonal:Western blot - SCN4B Rabbit pAb (A2154)}

    Western blot - SCN4B Rabbit pAb (A2154)

    Western blot analysis of various lysates using SCN4B Rabbit pAb (A2154) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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