제품 > 항체 > 다중클론항체(pAb)

SERPING1 Rabbit pAb (A1717)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SERPING1 Rabbit pAb (A1717)

Western blot analysis of extracts of Human plasma, using SERPING1 antibody (A1717) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - SERPING1 Rabbit pAb (A1717)

Immunofluorescence analysis of HeLa cells using SERPING1 Rabbit pAb (A1717) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SERPING1 Rabbit pAb (A1717)

Immunofluorescence analysis of HepG2 cells using SERPING1 Rabbit pAb (A1717) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameSERPING1 Rabbit pAb
Catalog No.A1717
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a highly glycosylated plasma protein involved in the regulation of the complement cascade. Its encoded protein, C1 inhibitor, inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. It is synthesized in the liver, and its deficiency is associated with hereditary angioneurotic oedema (HANE). Alternative splicing results in multiple transcript variants encoding the same isoform.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 201-500 of human SERPING1 (NP_001027466.1).
SequenceKDFTCVHQALKGFTTKGVTSVSQIFHSPDLAIRDTFVNASRTLYSSSPRVLSNNSDANLELINTWVAKNTNNKISRLLDSLPSDTRLVLLNAIYLSAKWKTTFDPKKTRMEPFHFKNSVIKVPMMNSKKYPVAHFIDQTLKAKVGQLQLSHNLSLVILVPQNLKHRLEDMEQALSPSVFKAIMEKLEMSKFQPTLLTLPRIKVTTSQDMLSIMEKLEFFDFSYDLNLCGLTEDPDLQVSAMQHQTVLELTETGVEAAAASAISVARTLLVFEVQQPFLFVLWDQQHKFPVFMGRVYDPRA
Gene ID
Swiss Prot
SynonymsC1IN; C1NH; HAE1; HAE2; C1INH
Calculated MW55kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHuman plasma
Cellular locationSecreted

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - SERPING1 Rabbit pAb (A1717)}

Western blot - SERPING1 Rabbit pAb (A1717)

Western blot analysis of extracts of Human plasma, using SERPING1 antibody (A1717) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - SERPING1 Rabbit pAb (A1717)}

Immunofluorescence - SERPING1 Rabbit pAb (A1717)

Immunofluorescence analysis of HeLa cells using SERPING1 Rabbit pAb (A1717) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SERPING1 Rabbit pAb (A1717)}

Immunofluorescence - SERPING1 Rabbit pAb (A1717)

Immunofluorescence analysis of HepG2 cells using SERPING1 Rabbit pAb (A1717) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

재조합 단백질 (1)

Secondary Antibodies (26)