제품 > 항체 > 단일클론항체(mAb)

SETD7 Rabbit mAb (A9225)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SETD7 Rabbit mAb (A9225)

Western blot analysis of various lysates using SETD7 Rabbit mAb (A9225) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Western blot - SETD7 Rabbit mAb (A9225)

Western blot analysis of lysates from HeLa cells, using SETD7 Rabbit mAb (A9225) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameSETD7 Rabbit mAb
Catalog No.A9225
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1483
Enables histone-lysine N-methyltransferase activity and p53 binding activity. Involved in peptidyl-lysine dimethylation and peptidyl-lysine monomethylation. Acts upstream of or within cellular response to DNA damage stimulus and heterochromatin organization. Located in chromosome and nucleolus.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human SETD7 (Q8WTS6).
SequenceMDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDT
Gene ID
Swiss Prot
SynonymsKMT7; SET7; SET9; SET7/9; SETD7
Calculated MW41kDa
Observed MW48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesC6, Mouse brain, Mouse spleen, Rat lung
Cellular locationChromosome, Nucleus
Customer validation

IF(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - SETD7 Rabbit mAb (A9225)}

Western blot - SETD7 Rabbit mAb (A9225)

Western blot analysis of various lysates using SETD7 Rabbit mAb (A9225) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Western blot - SETD7 Rabbit mAb (A9225)}

Western blot - SETD7 Rabbit mAb (A9225)

Western blot analysis of lysates from HeLa cells, using SETD7 Rabbit mAb (A9225) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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