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SH3PXD2A Rabbit pAb (A9363)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - SH3PXD2A Rabbit pAb (A9363)

Western blot analysis of lysates from HeLa cells, using SH3PXD2A Rabbit pAb (A9363).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - SH3PXD2A Rabbit pAb (A9363)

Immunofluorescence analysis of L929 cells using SH3PXD2A Rabbit pAb (A9363) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSH3PXD2A Rabbit pAb
Catalog No.A9363
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable superoxide-generating NADPH oxidase activator activity. Involved in osteoclast fusion and superoxide metabolic process. Located in podosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 806-1105 of human SH3PXD2A (NP_055446.2).
SequenceTKKEWEGPATSYMTCSAYQKVQDSEISFPAGVEVQVLEKQESGWWYVRFGELEGWAPSHYLVLDENEQPDPSGKELDTVPAKGRQNEGKSDSLEKIERRVQALNTVNQSKKATPPIPSKPPGGFGKTSGTPAVKMRNGVRQVAVRPQSVFVSPPPKDNNLSCALRRNESLTATDGLRGVRRNSSFSTARSAAAEAKGRLAERAASQGSDSPLLPAQRNSIPVSPVRPKPIEKSQFIHNNLKDVYVSIADYEGDEETAGFQEGVSMEVLERNPNGWWYCQILDGVKPFKGWVPSNYLEKKN
Gene ID
Swiss Prot
SynonymsFISH; TKS5; SH3MD1; SH3PXD2A
Calculated MW125kDa
Observed MW100kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa
Cellular locationCell projection, Cytoplasm, podosome
Customer validation

IF(Mus musculus)

WB(Homo sapiens)

Co-IP(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - SH3PXD2A Rabbit pAb (A9363)}

Western blot - SH3PXD2A Rabbit pAb (A9363)

Western blot analysis of lysates from HeLa cells, using SH3PXD2A Rabbit pAb (A9363).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - SH3PXD2A Rabbit pAb (A9363)}

Immunofluorescence - SH3PXD2A Rabbit pAb (A9363)

Immunofluorescence analysis of L929 cells using SH3PXD2A Rabbit pAb (A9363) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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