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SLC25A11 Rabbit pAb (A25022)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - SLC25A11 Rabbit pAb (A25022)

Western blot analysis of various lysates using SLC25A11 Rabbit pAb (A25022) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 45s.

Overview

Product nameSLC25A11 Rabbit pAb
Catalog No.A25022
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The oxoglutarate/malate carrier transports 2-oxoglutarate across the inner membranes of mitochondria in an electroneutral exchange for malate or other dicarboxylic acids (summary by Iacobazzi et al., 1992
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human SLC25A11(NP_003553.2).
SequenceMAATASAGAGGIDGKPRTSPKSVKFLFGGLAGMGATVFVQPLDLVKNRMQLSGEGAKTREYKTSFHALTSILKAEGLRGIYTGLSAGLLRQATYTTTRLG
Gene ID
Swiss Prot
SynonymsOGC; PGL6; SLC20A4; SLC25A11
Calculated MW34kDa
Observed MW34kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat, Mouse liver
Cellular locationMitochondrion inner membrane, Multi-pass membrane protein
Customer validation

WB(Homo sapiens, Mus musculus)

Co-IP(Homo sapiens, Mus musculus)

RT-PCR(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - SLC25A11 Rabbit pAb (A25022)}

    Western blot - SLC25A11 Rabbit pAb (A25022)

    Western blot analysis of various lysates using SLC25A11 Rabbit pAb (A25022) at 1:1000 dilution.
    Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
    Lysates / proteins: 25 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection:ECL Basic Kit (RM00020).
    Exposuretime: 45s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A25022? Please let us know so that we can cite the reference in this datasheet.

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