제품 > 항체 > 다중클론항체(pAb)

SLR1 Rabbit pAb (A14614)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Oryza sativa

Western blot analysis of recombinant Oryza sativa SLR1 protein using SLR1 Rabbit pAb (A14614).
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 10ng/5ng/1ng/500pg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Western blot analysis of various lysates using SLR1 Rabbit pAb (A14614) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:8000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product name	SLR1 Rabbit pAb
Catalog No.	A14614
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway. In contrast, its overexpression prevents the GA signaling pathway and induces a dwarf phenotype.

Immunogen information

Immunogen	Recombinant protein of Oryza sativa SLR1
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	GAI; SLR1; OsGAI; OsJ_12286
Calculated MW	65kDa
Observed MW	65.4kDa

Applications

Reactivity	Oryza sativa
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Rice root, Rice leaf, Recombinant Oryza sativa SLR1 protein
Cellular location
Customer validation	WB(Oryza sativa) IP(Oryza sativa)