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SOX1 Rabbit pAb (A3086)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SOX1 Rabbit pAb (A3086)

Western blot analysis of various lysates using SOX1 Rabbit pAb (A3086) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - SOX1 Rabbit pAb (A3086)

Immunohistochemistry analysis of paraffin-embedded mouse embryos using SOX1 Rabbit pAb (A3086) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameSOX1 Rabbit pAb
Catalog No.A3086
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. In mice, a similar protein regulates the gamma-crystallin genes and is essential for lens development.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 292-391 of human SOX1 (NP_005977.2).
SequenceHQNSAVAAAAAAAAASSGALGALGSLVKSEPSGSPPAPAHSRAPCPGDLREMISMYLPAGEGGDPAAAAAAAAQSRLHSLPQHYQGAGAGVNGTVPLTHI
Gene ID
Swiss Prot
SynonymsSOX1; SRY-box 1
Calculated MW39kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse brain, Rat ovary
Cellular locationNucleus

Documents

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ABclonal:Western blot - SOX1 Rabbit pAb (A3086)}

Western blot - SOX1 Rabbit pAb (A3086)

Western blot analysis of various lysates using SOX1 Rabbit pAb (A3086) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - SOX1 Rabbit pAb (A3086)}

Immunohistochemistry - SOX1 Rabbit pAb (A3086)

Immunohistochemistry analysis of paraffin-embedded mouse embryos using SOX1 Rabbit pAb (A3086) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)