제품 > 항체 > 다중클론항체(pAb)

SP7 Rabbit pAb (A18699)

Publications (15) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SP7 Rabbit pAb (A18699)

Western blot analysis of extracts of U2OS cells, using SP7 antibody (A18699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Western blot - SP7 Rabbit pAb (A18699)

Western blot analysis of extracts of Mouse bone, using SP7 antibody (A18699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Western blot - SP7 Rabbit pAb (A18699)

Western blot analysis of extracts of Rat brain, using SP7 antibody (A18699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product nameSP7 Rabbit pAb
Catalog No.A18699
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein is a bone specific transcription factor and is required for osteoblast differentiation and bone formation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 333-376 of human SP7 (NP_001166938.1).
SequenceKRFTRSDELERHVRTHTREKKFTCLLCSKRFTRSDHLSKHQRTH
Gene ID
Swiss Prot
SynonymsOSX; OI11; OI12; osterix
Calculated MW45kDa
Observed MW45kDa/46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU2OS, Mouse bone, Rat brain
Cellular locationcytoplasm, nucleus
Customer validation

WB(Mus musculus, Mus musculus , Rattus norvegicus)

IHC(Mus musculus)

RT-PCR(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - SP7 Rabbit pAb (A18699)}

Western blot - SP7 Rabbit pAb (A18699)

Western blot analysis of extracts of U2OS cells, using SP7 antibody (A18699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Western blot - SP7 Rabbit pAb (A18699)}

Western blot - SP7 Rabbit pAb (A18699)

Western blot analysis of extracts of Mouse bone, using SP7 antibody (A18699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Western blot - SP7 Rabbit pAb (A18699)}

Western blot - SP7 Rabbit pAb (A18699)

Western blot analysis of extracts of Rat brain, using SP7 antibody (A18699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A18699? Please let us know so that we can cite the reference in this datasheet.