Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of extracts of U2OS cells, using SP7 antibody (A18699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Western blot analysis of extracts of Mouse bone, using SP7 antibody (A18699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Western blot analysis of extracts of Rat brain, using SP7 antibody (A18699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
| Product name | SP7 Rabbit pAb |
|---|---|
| Catalog No. | A18699 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 333-376 of human SP7 (NP_001166938.1). |
|---|---|
| Sequence | KRFTRSDELERHVRTHTREKKFTCLLCSKRFTRSDHLSKHQRTH |
| Gene ID | |
| Swiss Prot | |
| Synonyms | OSX; OI11; OI12; osterix |
| Calculated MW | 45kDa |
| Observed MW | 45kDa/46kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | U2OS, Mouse bone, Rat brain |
| Cellular location | cytoplasm, nucleus |
| Customer validation | WB(Mus musculus, Mus musculus , Rattus norvegicus) IHC(Mus musculus) RT-PCR(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A18699? Please let us know so that we can cite the reference in this datasheet.
