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SRPX Rabbit pAb (A1217)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - SRPX Rabbit pAb (A1217)

Western blot analysis of lysates from Mouse heart, using SRPX Rabbit pAb (A1217) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - SRPX Rabbit pAb (A1217)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using SRPX Rabbit pAb (A1217) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameSRPX Rabbit pAb
Catalog No.A1217
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be an extracellular matrix structural constituent. Predicted to be involved in cell adhesion. Predicted to act upstream of or within several processes, including negative regulation of cell proliferation involved in contact inhibition; phagolysosome assembly; and positive regulation of extrinsic apoptotic signaling pathway in absence of ligand. Part of collagen-containing extracellular matrix.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 40-320 of human SRPX (NP_006298.1).
SequenceEDDEVGYSHPRYKDTPWCSPIKVKYGDVYCRAPQGGYYKTALGTRCDIRCQKGYELHGSSLLICQSNKRWSDKVICKQKRCPTLAMPANGGFKCVDGAYFNSRCEYYCSPGYTLKGERTVTCMDNKAWSGRPASCVDMEPPRIKCPSVKERIAEPNKLTVRVSWETPEGRDTADGILTDVILKGLPPGSNFPEGDHKIQYTVYDRAENKGTCKFRVKVRVKRCGKLNAPENGYMKCSSDGDNYGATCEFSCIGGYELQGSPARVCQSNLAWSGTEPTCAAM
Gene ID
Swiss Prot
SynonymsDRS; ETX1; SRPX1; HEL-S-83p; SRPX
Calculated MW52kDa
Observed MW35kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse heart
Cellular locationCell surface
Customer validation

IHC(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - SRPX Rabbit pAb (A1217)}

    Western blot - SRPX Rabbit pAb (A1217)

    Western blot analysis of lysates from Mouse heart, using SRPX Rabbit pAb (A1217) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - SRPX Rabbit pAb (A1217)}

    Immunohistochemistry - SRPX Rabbit pAb (A1217)

    Immunohistochemistry analysis of paraffin-embedded mouse kidney using SRPX Rabbit pAb (A1217) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A1217? Please let us know so that we can cite the reference in this datasheet.