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SUCLA2 Rabbit pAb (A10040)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SUCLA2 Rabbit pAb (A10040)

Western blot analysis of extracts of various cell lines, using SUCLA2 antibody (A10040) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - SUCLA2 Rabbit pAb (A10040)

Immunofluorescence analysis of L929 using SUCLA2 antibody (A10040) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SUCLA2 Rabbit pAb (A10040)

Immunofluorescence analysis of U2OS using SUCLA2 antibody (A10040) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameSUCLA2 Rabbit pAb
Catalog No.A10040
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human SUCLA2 (NP_003841.1).
SequenceMAASMFYGRLVAVATLRNHRPRTAQRAAAQVLGSSGLFNNHGLQVQQQQQRNLSLHEYMSMELLQEAGVSVPKGYVAKSPDEAYAIAKKLGSKDVVIKAQVLAGGRGKGTFESGLKGGVKIVFSPEEAKAVSSQMIGKKLFTKQTGEKGRICNQVLVCERKYPRREYYFAITMERSFQGP
Gene ID
Swiss Prot
SynonymsA-SCS; A-BETA; MTDPS5; LINC00444; SCS-betaA
Calculated MW50kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, SW480, HeLa, Mouse heart, Mouse liver, Mouse brain, Rat liver
Cellular locationMitochondrion

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ABclonal:Western blot - SUCLA2 Rabbit pAb (A10040)}

Western blot - SUCLA2 Rabbit pAb (A10040)

Western blot analysis of extracts of various cell lines, using SUCLA2 antibody (A10040) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - SUCLA2 Rabbit pAb (A10040)}

Immunofluorescence - SUCLA2 Rabbit pAb (A10040)

Immunofluorescence analysis of L929 using SUCLA2 antibody (A10040) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SUCLA2 Rabbit pAb (A10040)}

Immunofluorescence - SUCLA2 Rabbit pAb (A10040)

Immunofluorescence analysis of U2OS using SUCLA2 antibody (A10040) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.