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SYT1 Rabbit pAb (A24840)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - SYT1 Rabbit pAb (A24840)

Western blot analysis of lysates from Mouse brain using SYT1 Rabbit pAb(A24840) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Overview

Product nameSYT1 Rabbit pAb
Catalog No.A24840
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human SYT1(NP_005630.1).
SequenceMVSESHHEALAAPPVTTVATVLPSNATEPASPGEGKEDAFSKLKEKFMNELHKIPLPPWALIAIAIVAVLLVLTCCFCICKKCLFKKKNKKKGKEKGGKN
Gene ID
Swiss Prot
SynonymsSYT1; P65; SVP65; SYT; synaptotagmin-1
Calculated MW47kDa
Observed MW60kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain
Cellular locationCytoplasm, Cytoplasmic vesicle, Single-pass membrane protein, chromaffin granule membrane, secretory vesicle, synaptic vesicle membrane

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ABclonal:Western blot - SYT1 Rabbit pAb (A24840)}

Western blot - SYT1 Rabbit pAb (A24840)

Western blot analysis of lysates from Mouse brain using SYT1 Rabbit pAb(A24840) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)