제품 > 항체 > 다중클론항체(pAb)

Septin 3 Rabbit pAb (A16551)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Septin 3 Rabbit pAb (A16551)

Western blot analysis of various lysates using Septin 3 Rabbit pAb (A16551) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - Septin 3 Rabbit pAb (A16551)

Immunofluorescence analysis of L929 cells using Septin 3 Rabbit pAb (A16551) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Septin 3 Rabbit pAb (A16551)

Immunofluorescence analysis of U-2 OS cells using Septin 3 Rabbit pAb (A16551) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSeptin 3 Rabbit pAb
Catalog No.A16551
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in several transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 179-358 of human Septin 3 (NP_663786.2).
SequenceSPTGHSLRPLDLEFMKHLSKVVNIIPVIAKADTMTLEEKSEFKQRVRKELEVNGIEFYPQKEFDEDLEDKTENDKIRQESMPFAVVGSDKEYQVNGKRVLGRKTPWGIIEVENLNHCEFALLRDFVIRTHLQDLKEVTHNIHYETYRAKRLNDNGGLPPGEGLLGTVLPPVPATPCPTAE
Gene ID
Swiss Prot
SynonymsSEP3; SEPT3; bK250D10.3; Septin 3
Calculated MW41kDa
Observed MW41kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse brain, Rat brain
Cellular locationCell junction, Cytoplasm, cytoskeleton, synapse

Documents

Certificate of Compliance

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    ABclonal:Western blot - Septin 3 Rabbit pAb (A16551)}

    Western blot - Septin 3 Rabbit pAb (A16551)

    Western blot analysis of various lysates using Septin 3 Rabbit pAb (A16551) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - Septin 3 Rabbit pAb (A16551)}

    Immunofluorescence - Septin 3 Rabbit pAb (A16551)

    Immunofluorescence analysis of L929 cells using Septin 3 Rabbit pAb (A16551) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Septin 3 Rabbit pAb (A16551)}

    Immunofluorescence - Septin 3 Rabbit pAb (A16551)

    Immunofluorescence analysis of U-2 OS cells using Septin 3 Rabbit pAb (A16551) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.