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Smac/Diablo Rabbit mAb (A8889)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Smac/Diablo Rabbit mAb (A8889)

Western blot analysis of various lysates using Smac/Diablo Rabbit mAb (A8889) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Smac/Diablo Rabbit mAb (A8889)

Immunohistochemistry analysis of paraffin-embedded Rat testis using Smac/Diablo Rabbit mAb (A8889) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Smac/Diablo Rabbit mAb (A8889)

Immunofluorescence analysis of C6 cells using Smac/Diablo Rabbit mAb (A8889) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Smac/Diablo Rabbit mAb (A8889)

Immunofluorescence analysis of NIH/3T3 cells using Smac/Diablo Rabbit mAb (A8889) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Smac/Diablo Rabbit mAb (A8889)

Immunofluorescence analysis of U2OS cells using Smac/Diablo Rabbit mAb (A8889) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSmac/Diablo Rabbit mAb
Catalog No.A8889
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1340
This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 56-239 of human Smac/Diablo (NP_063940.1).
SequenceAVPIAQKSEPHSLSSEALMRRAVSLVTDSTSTFLSQTTYALIEAITEYTKAVYTLTSLYRQYTSLLGKMNSEEEDEVWQVIIGARAEMTSKHQEYLKLETTWMTAVGLSEMAAEAAYQTGADQASITARNHIQLVKLQVEEVHQLSRKAETKLAEAQIEELRQKTQEEGEERAESEQEAYLRED
Gene ID
Swiss Prot
SynonymsSMAC; DFNA64; Smac/Diablo
Calculated MW27kDa/21kDa/22kDa
Observed MW19kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSP2/0, HeLa, MCF7, C2C12, Jurkat, C6, Mouse heart, Rat lung, Rat testis
Cellular locationMitochondrion

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Smac/Diablo Rabbit mAb (A8889)}

Western blot - Smac/Diablo Rabbit mAb (A8889)

Western blot analysis of various lysates using Smac/Diablo Rabbit mAb (A8889) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Smac/Diablo Rabbit mAb (A8889)}

Immunohistochemistry - Smac/Diablo Rabbit mAb (A8889)

Immunohistochemistry analysis of paraffin-embedded Rat testis using Smac/Diablo Rabbit mAb (A8889) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Smac/Diablo Rabbit mAb (A8889)}

Immunofluorescence - Smac/Diablo Rabbit mAb (A8889)

Immunofluorescence analysis of C6 cells using Smac/Diablo Rabbit mAb (A8889) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Smac/Diablo Rabbit mAb (A8889)}

Immunofluorescence - Smac/Diablo Rabbit mAb (A8889)

Immunofluorescence analysis of NIH/3T3 cells using Smac/Diablo Rabbit mAb (A8889) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Smac/Diablo Rabbit mAb (A8889)}

Immunofluorescence - Smac/Diablo Rabbit mAb (A8889)

Immunofluorescence analysis of U2OS cells using Smac/Diablo Rabbit mAb (A8889) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)