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TBL1XR1 Rabbit pAb (A13438)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - TBL1XR1 Rabbit pAb (A13438)

Western blot analysis of lysates from K-562 cells, using TBL1XR1 Rabbit pAb (A13438) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - TBL1XR1 Rabbit pAb (A13438)

Western blot analysis of lysates from Mouse brain, using TBL1XR1 Rabbit pAb (A13438) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunofluorescence - TBL1XR1 Rabbit pAb (A13438)

Immunofluorescence analysis of A549 cells using TBL1XR1 Rabbit pAb (A13438).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

Overview

Product nameTBL1XR1 Rabbit pAb
Catalog No.A13438
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the WD40 repeat-containing gene family and shares sequence similarity with transducin (beta)-like 1X-linked (TBL1X). The protein encoded by this gene is thought to be a component of both nuclear receptor corepressor (N-CoR) and histone deacetylase 3 (HDAC 3) complexes, and is required for transcriptional activation by a variety of transcription factors. Mutations in these gene have been associated with some autism spectrum disorders, and one finding suggests that haploinsufficiency of this gene may be a cause of intellectual disability with dysmorphism. Mutations in this gene as well as recurrent translocations involving this gene have also been observed in some tumors.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 120-170 of human TBL1XR1 (NP_078941.2).
SequenceQQGSAKNGENTANGEENGAHTIANNHTDMMEVDGDVEIPPNKAVVLRGHES
Gene ID
Swiss Prot
SynonymsC21; DC42; IRA1; MRD41; TBLR1; TBL1XR1
Calculated MW56kDa
Observed MW60kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesK-562, Mouse brain
Cellular locationNucleus.

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ABclonal:Western blot - TBL1XR1 Rabbit pAb (A13438)}

Western blot - TBL1XR1 Rabbit pAb (A13438)

Western blot analysis of lysates from K-562 cells, using TBL1XR1 Rabbit pAb (A13438) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - TBL1XR1 Rabbit pAb (A13438)}

Western blot - TBL1XR1 Rabbit pAb (A13438)

Western blot analysis of lysates from Mouse brain, using TBL1XR1 Rabbit pAb (A13438) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunofluorescence - TBL1XR1 Rabbit pAb (A13438)}

Immunofluorescence - TBL1XR1 Rabbit pAb (A13438)

Immunofluorescence analysis of A549 cells using TBL1XR1 Rabbit pAb (A13438).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

* For research use only. Not for therapeutic or diagnostic purposes.

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