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TBRG4 Rabbit pAb (A15753)

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Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TBRG4 Rabbit pAb (A15753)

Western blot analysis of lysates from HepG2 cells, using TBRG4 Rabbit pAb (A15753) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - TBRG4 Rabbit pAb (A15753)

Immunofluorescence analysis of L929 cells using TBRG4 Rabbit pAb (A15753) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - TBRG4 Rabbit pAb (A15753)

Immunofluorescence analysis of U-2 OS cells using TBRG4 Rabbit pAb (A15753) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameTBRG4 Rabbit pAb
Catalog No.A15753
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables RNA binding activity. Involved in mitochondrial mRNA processing and regulation of mitochondrial mRNA stability. Located in mitochondrial matrix.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 382-631 of human TBRG4 (NP_004740.2).
SequenceHPDQEDQFFSLVHEKLGSELPGLEPALQVDLVWALCVLQQAREAELQAVLHPEFHIQFLGGKSQKDQNTFQKLLHINATALLEYPEYSGPLLPASAVAPGPSALDRKVTPLQKELQETLKGLLGSADKGSLEVATQYGWVLDAEVLLDSDGEFLPVRDFVAPHLAQPTGSQSPPPGSKRLAFLRWEFPNFNSRSKDLLGRFVLARRHIVAAGFLIVDVPFYEWLELKSEWQKGAYLKDKMRKAVAEELAK
Gene ID
Swiss Prot
SynonymsCPR2; FASTKD4; TBRG4
Calculated MW71kDa
Observed MW68-71kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHepG2
Cellular locationMitochondrion.

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ABclonal:Western blot - TBRG4 Rabbit pAb (A15753)}

Western blot - TBRG4 Rabbit pAb (A15753)

Western blot analysis of lysates from HepG2 cells, using TBRG4 Rabbit pAb (A15753) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - TBRG4 Rabbit pAb (A15753)}

Immunofluorescence - TBRG4 Rabbit pAb (A15753)

Immunofluorescence analysis of L929 cells using TBRG4 Rabbit pAb (A15753) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TBRG4 Rabbit pAb (A15753)}

Immunofluorescence - TBRG4 Rabbit pAb (A15753)

Immunofluorescence analysis of U-2 OS cells using TBRG4 Rabbit pAb (A15753) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.