제품 > 항체 > 다중클론항체(pAb)

TIMP1 Rabbit pAb (A1389)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using TIMP1 Rabbit pAb (A1389) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - TIMP1 Rabbit pAb (A1389)

Immunofluorescence analysis of HeLa cells using TIMP1 Rabbit pAb (A1389) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	TIMP1 Rabbit pAb
Catalog No.	A1389
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 128-207 of human TIMP1 (NP_003245.1).
Sequence	WNSLSLAQRRGFTKTYTVGCEECTVFPCLSIPCKLQSGTHCLWTDQLLQGSEKGFQSRHLACLPREPGLCTWQSLRSQIA
Gene ID
Swiss Prot
Synonyms	EPA; EPO; HCI; CLGI; TIMP; TIMP-1; TIMP1
Calculated MW	23kDa
Observed MW	28kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa, Rat uterus
Cellular location	Secreted.
Customer validation	WB(Mus musculus, Oryctolagus cuniculus, Homo sapiens, Rattus norvegicus) IF(Homo sapiens) WB(Homo sapiens)

Documents

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Research Area

TIMP1 Rabbit pAb images

Western blot - TIMP1 Rabbit pAb (A1389)

Immunofluorescence - TIMP1 Rabbit pAb (A1389)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for TIMP1 Rabbit pAb (A1389)

Product:

TIMP1 Rabbit pAb

Journal:

Exp Physiol

Application:

IF:

2.7

Species:

Mus musculus

PMID:

32936966

Title:

IGFBP3 inhibits angiotensin II‐induced aortic smooth muscle cell phenotypic switch and MMPs expression

References for TIMP1 Rabbit pAb (A1389)

Product:

TIMP1 Rabbit pAb

Journal:

BMC Med Genomics

Application:

IF:

3.6

Species:

Homo sapiens

PMID:

34587952

Title:

Identification and validation of pivotal genes related to age-related meniscus degeneration based on gene expression profiling analysis and in vivo and in vitro models detection

References for TIMP1 Rabbit pAb (A1389)

Product:

TIMP1 Rabbit pAb

Journal:

Bioact Mater

Application:

IF:

18.9

Species:

Oryctolagus cuniculus

PMID:

34901552

Title:

A functional PVA aerogel-based membrane obtaining sutureability through modified electrospinning technology and achieving promising anti-adhesion effect after cardiac surgery

References for TIMP1 Rabbit pAb (A1389)

Product:

TIMP1 Rabbit pAb

Journal:

Front Endocrinol (Lausanne)

Application:

IF:

3.9

Species:

Homo sapiens

PMID:

34777244

Title:

The Imbalance of MMP-2/TIMP-2 and MMP-9/TIMP-1 Contributes to Collagen Deposition Disorder in Diabetic Non-Injured Skin

References for TIMP1 Rabbit pAb (A1389)

Product:

TIMP1 Rabbit pAb

Journal:

Tissue & cell

Application:

IF:

2.7

Species:

Homo sapiens

PMID:

36156375

Title:

CCL26 silence represses colon cancer by inhibiting the EMT signaling pathway

References for TIMP1 Rabbit pAb (A1389)

Product:

TIMP1 Rabbit pAb

Journal:

The Science of the total environment

Application:

IF:

8.2

Species:

Mus musculus

PMID:

35597360

Title:

Oxidative stress mediated by the TLR4/NOX2 signalling axis is involved in polystyrene microplastic-induced uterine fibrosis in mice

References for TIMP1 Rabbit pAb (A1389)

Product:

TIMP1 Rabbit pAb

Journal:

Connective tissue research

Application:

IF:

2.8

Species:

Rattus norvegicus

PMID:

37092609

Title:

Ovariectomy with simulated vaginal delivery to establish a rat model for pelvic organ prolapse

References for TIMP1 Rabbit pAb (A1389)

Product:

TIMP1 Rabbit pAb

Journal:

Chinese medical journal

Application:

IF:

7.5

Species:

Homo sapiens

PMID:

36914936

Title:

Telomerase-mediated immortalization of human vaginal wall fibroblasts derived from patients with pelvic organ prolapse

References for TIMP1 Rabbit pAb (A1389)

Product:

TIMP1 Rabbit pAb

Journal:

Lasers in medical science

Application:

IF:

2.1

Species:

Homo sapiens

PMID:

37819407

Title:

Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress - PMC

References for TIMP1 Rabbit pAb (A1389)

Product:

TIMP1 Rabbit pAb

Journal:

Immunity, inflammation and disease

Application:

IF:

2.5

Species:

Rattus norvegicus

PMID:

38156377

Title:

Mechanism of Rhodiola rosea-Euonymus alatus drug pair against rheumatoid arthritis: Network pharmacology and experimental validation - PMC

References for TIMP1 Rabbit pAb (A1389)

Product:

TIMP1 Rabbit pAb

Journal:

Brain research

Application:

IF:

2.7

Species:

Mus musculus

PMID:

27328426

Title:

Hyperforin promotes post-stroke functional recovery through interleukin (IL) 17A-mediated angiogenesis

References for TIMP1 Rabbit pAb (A1389)

Product:

TIMP1 Rabbit pAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Rattus norvegicus

PMID:

39363196

Title:

Modification of transvaginal polypropylene mesh with co-axis electrospun nanofibrous membrane to alleviate complications following surgical implantation - PMC

References for TIMP1 Rabbit pAb (A1389)

Product:

TIMP1 Rabbit pAb

Journal:

Experimental eye research

Application:

IF, WB

IF:

Species:

Homo sapiens

PMID:

38272381